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Rabbit Anti-CD90/Thy-1 antibody (bs-0778R)
訂購熱線:400-901-9800
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訂購傳真:010-58129612
技術支持:[email protected]
說明書: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
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產品編號 bs-0778R
英文名稱 CD90/Thy-1
中文名稱 CD90抗體
別    名 CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.  
Specific References  (19)     |     bs-0778R has been referenced in 19 publications.
[IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900  FCM ;  Human.  
[IF=2.66] Li et al. Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway. (2016) Oncol.Rep. 36:936-44  IF(ICC) ;  Rat.  
[IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.  
[IF=1.461] Pei?W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.  
[IF=4.963] Jin J et al. Exosome?secreted?from?adipose-derived?stem?cells?attenuates?diabetic?nephropathy?by?promoting?autophagy?flux?and?inhibiting?apoptosis?in?podocyte.Stem?Cell Res Ther.?2019 Mar 15;10(1):95.?  ICF ;  Human.  
[IF=.52] Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.  IF(ICC) ;  Rat.  
[IF=0] Gstraunthaler, Gerhard, et al. "Human platelet lysates successfully replace fetal bovine serum in adipose-derived adult stem cell culture." Journal of Advanced Biotechnology and Bioengineering 2.1 (2014): 1-11.  IF(ICC) ;  Human.  
[IF=2.26] Yu, Xian-huan, et al. "Clinicopathological characteristics of 20 cases of hepatocellular carcinoma with bile duct tumor thrombi." Digestive diseases and sciences 56.1 (2011): 252-259.  IHC-P ;  Human.  
[IF=1.43] Zeng, Biao, et al. "Increased expression of importin13 in endometriosis and endometrial carcinoma." Medical Science Monitor 18.6 (2012): CR361-CR367.  IHC-P ;  Human.  
[IF=1.06] Scott, Erin M., et al. "Early histopathologic changes in the retina and optic nerve in canine primary angle‐closure glaucoma." Veterinary ophthalmology16.s1 (2013): 79-86.  IHC-P ;  Dog.  
[IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).  Rat.  
[IF=2.94] Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013).  FCM ;  Rat.  
[IF=5.9] Chen, Guobao, et al. "3D Scaffolds with Different Stiffness but Same Microstructure for Bone Tissue Engineering." ACS Applied Materials & Interfaces (2015).  IHC-F ;  Rat.  
[IF=3.36] Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).  Rat.  
[IF=1.52] Tepekoy, Filiz, et al. "CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development." Reproductive Biology(2015).  IHC-P ;  Mouse.  
[IF=1.8] Vansandt, Lindsey M., et al. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology(2016).  IHC-P ;  Other Species.  
[IF=1.92] Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).  FCM ;  Sheep.  
[IF=4.034] Wang YL et al. Preinduction with bone morphogenetic protein-2 enhances cardiomyogenic differentiation of c-kit+ mesenchymal stem cells and repair of infarcted myocardium.Int J Cardiol. 2018 Aug 15;265:173-180.  FCM ;  Rat.  
[IF=.918] Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224.  ICF ;  lamb.  
研究領域 細胞生物  免疫學  神經生物學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, Sheep, )
產品應用 WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 12kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from rat Thy-1:31-120/161 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Abundant in lymphoid tissues.

Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01830

Gene ID:
24832

Database links:

Entrez Gene: 7070 Human

Entrez Gene: 21838 Mouse

Entrez Gene: 24832 Rat

Omim: 188230 Human

SwissProt: P04216 Human

SwissProt: P01831 Mouse

SwissProt: P01830 Rat

Unigene: 644697 Human

Unigene: 3951 Mouse

Unigene: 108198 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Thy-1/CD90/ Thy1.1 Polyclonal Antibody, Unconjugated(bs-0778R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Mouse brain.
Primary Antibody (green line): Rabbit Anti-CD90 antibody (bs-0778R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse brain.
Primary Antibody (green line): Rabbit Anti-CD90 antibody (bs-0778R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-CD90 antibody(bs-0778R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-0778R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti- CD90 antibody (bs-0778R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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